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non essential amino acids neaa  (Thermo Fisher)


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    Thermo Fisher non essential amino acids neaa
    Non Essential Amino Acids Neaa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-essential+amino+acids+(neaa/2'%2C7'-Dichlorofluorescein/pm41227604-42-15-31
    Average 99 stars, based on 1 article reviews
    non essential amino acids neaa - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The Impact of Amotosalen Photochemical Pathogen Inactivation on Human Platelet Lysate
    Article Snippet: Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium; Transfusion Innovation Center, Belgian Red Cross Flanders, Ghent, Belgium; Blood Services, Belgian Red Cross Flanders, Mechelen, Belgium; Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine and Health Sciences, Gent University, Gent, Belgium; Department of Public Health and Primary Care, Faculty of Medicine, KU Leuven, Leuven, Belgium

    Article Title: Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication
    Article Snippet: After day 9 of differentiation, cells were cultured in a medium consisting of DMEM/F12 containing 15% (v/v) Knock-Out Serum Replacement (KSR, Thermo Fisher Scientific), 1:100 (v/v) non-essential amino acids (NEAA, Thermo Fisher Scientific), 1:100 (v/v) penicillin-streptomycin (P/S, Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol (Sigma), 10 ng/mL HGF (Peprotech), and 2 ng/mL IGF-1 (Peprotech).

    Article Title: Imidazolium salt’s toxic effects in larvae and cells of Aedes aegypti and Aedes albopictus (Diptera: Culicidae)
    Article Snippet: All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM – Gibco, Thermo Fischer Scientific, Massachusetts, USA) with low levels of glucose, supplemented with 1% non-essential amino acids (NEAA – Gibco, Thermo Fischer Scientific, Massachusetts, USA), maintained at 28 °C in a 5% CO 2 incubator.

    Article Title: Proof of stability of an RSV Controlled Human Infection Model challenge agent.
    Article Snippet: Briefly, HEp2 cells (ATCC CCL-23) grown in Dulbecco’s Minimum Essential Medium (DMEM; Gibco/Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum (FBS, Tico Europe, Amstelveen, The Netherlands), 2 mM L-glutamine (Gibco/Thermo Fisher Scientific) and 1% Non-Essential Amino Acids (NEAA; Gibco/Thermo Fisher Scientific) were seeded (5 × 103 cells/well) in HEp2 medium in 96-well plates (Greiner Bio-One, Vilvoorde, Belgium) and grown until reaching 80–90% confluence.

    Article Title: Antimicrobial and immunomodulatory activities of porcine cathelicidin Protegrin-1.
    Article Snippet: Promastigotes were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal calf serum (FCS) (Bodinco B.V., Alkmaar, The Netherlands), 1x non-essential amino acids (NEAA) (Gibco), 100 units/ mL Penicillin, 100 μg/mL Streptomycin, 10 μg/mL Hemin and 10 μM Xanthine (all Sigma) at 26 ◦C.

    Article Title: Cryopreservative compositions and methods
    Article Snippet: MSCs were cultured in alpha-MEM (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with non-essential amino acids (NEAA, Gibco, Thermo Fisher Scientific, Waltham, MA) and 10% FBS (qualified, Gibco, Thermo Fisher Scientific, Waltham, MA) in a 37° C. incubator at 5% CO2.

    Article Title: Computational analysis of zoanthamine alkaloids from Zoanthus sp. as potential DKK1 and GSK-3β inhibitors for osteoporosis therapy via Wnt signaling.
    Article Snippet: The reagents and chemicals employed for cell culture comprised Minimum Essential Medium α (MEM α, Thermo Scientific, Waltham, USA), fetal bovine serum (FBS, Merck, Darmstadt, Germany), penicillin-streptomycin (Thermo Scientific, Waltham, USA), sodium pyruvate (Thermo Scientific, Waltham, USA), non-essential amino acids (NEAA, Thermo Scientific, Waltham, USA), sodium bicarbonate (Sigma-Aldrich, St. Louis, USA), trypan blue (Sigma-Aldrich, St. Louis, USA), Dulbecco’s phosphate-buffered saline (PBS, Gibco, Waltham, USA), and trypsin (Thermo Scientific, Waltham, USA).

    Article Title: In vivo affinity maturation of the Env-binding domains of the HIV-1 entry inhibitor CD4-Ig
    Article Snippet: Before electroporation, B cells were activated for 36–42 hours in RPMI 1640 medium with 10% FBS, 100 μM Non-Essential Amino Acids (NEAA, Thermo Fisher Scientific, 11140050), 1 mM sodium pyruvate (Thermo Fisher Scientific, 11360070), 10 mM HEPES (Thermo Fisher Scientific, 15630080), 55 μM 2-Mercaptoethanol (Thermo Fisher Scientific, 21985023), 100 units/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific, 15140163), and 5 μg/ml anti-mouse CD180 antibody (Biolegend, 117708).

    Knock-Out:

    Article Title: The Impact of Amotosalen Photochemical Pathogen Inactivation on Human Platelet Lysate
    Article Snippet: Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium; Transfusion Innovation Center, Belgian Red Cross Flanders, Ghent, Belgium; Blood Services, Belgian Red Cross Flanders, Mechelen, Belgium; Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine and Health Sciences, Gent University, Gent, Belgium; Department of Public Health and Primary Care, Faculty of Medicine, KU Leuven, Leuven, Belgium

    Article Title: Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication
    Article Snippet: After day 9 of differentiation, cells were cultured in a medium consisting of DMEM/F12 containing 15% (v/v) Knock-Out Serum Replacement (KSR, Thermo Fisher Scientific), 1:100 (v/v) non-essential amino acids (NEAA, Thermo Fisher Scientific), 1:100 (v/v) penicillin-streptomycin (P/S, Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol (Sigma), 10 ng/mL HGF (Peprotech), and 2 ng/mL IGF-1 (Peprotech).

    Article Title: Imidazolium salt’s toxic effects in larvae and cells of Aedes aegypti and Aedes albopictus (Diptera: Culicidae)
    Article Snippet: All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM – Gibco, Thermo Fischer Scientific, Massachusetts, USA) with low levels of glucose, supplemented with 1% non-essential amino acids (NEAA – Gibco, Thermo Fischer Scientific, Massachusetts, USA), maintained at 28 °C in a 5% CO 2 incubator.

    Article Title: Proof of stability of an RSV Controlled Human Infection Model challenge agent.
    Article Snippet: Briefly, HEp2 cells (ATCC CCL-23) grown in Dulbecco’s Minimum Essential Medium (DMEM; Gibco/Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum (FBS, Tico Europe, Amstelveen, The Netherlands), 2 mM L-glutamine (Gibco/Thermo Fisher Scientific) and 1% Non-Essential Amino Acids (NEAA; Gibco/Thermo Fisher Scientific) were seeded (5 × 103 cells/well) in HEp2 medium in 96-well plates (Greiner Bio-One, Vilvoorde, Belgium) and grown until reaching 80–90% confluence.

    Article Title: Antimicrobial and immunomodulatory activities of porcine cathelicidin Protegrin-1.
    Article Snippet: Promastigotes were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal calf serum (FCS) (Bodinco B.V., Alkmaar, The Netherlands), 1x non-essential amino acids (NEAA) (Gibco), 100 units/ mL Penicillin, 100 μg/mL Streptomycin, 10 μg/mL Hemin and 10 μM Xanthine (all Sigma) at 26 ◦C.

    Article Title: Cryopreservative compositions and methods
    Article Snippet: MSCs were cultured in alpha-MEM (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with non-essential amino acids (NEAA, Gibco, Thermo Fisher Scientific, Waltham, MA) and 10% FBS (qualified, Gibco, Thermo Fisher Scientific, Waltham, MA) in a 37° C. incubator at 5% CO2.

    Article Title: Computational analysis of zoanthamine alkaloids from Zoanthus sp. as potential DKK1 and GSK-3β inhibitors for osteoporosis therapy via Wnt signaling.
    Article Snippet: The reagents and chemicals employed for cell culture comprised Minimum Essential Medium α (MEM α, Thermo Scientific, Waltham, USA), fetal bovine serum (FBS, Merck, Darmstadt, Germany), penicillin-streptomycin (Thermo Scientific, Waltham, USA), sodium pyruvate (Thermo Scientific, Waltham, USA), non-essential amino acids (NEAA, Thermo Scientific, Waltham, USA), sodium bicarbonate (Sigma-Aldrich, St. Louis, USA), trypan blue (Sigma-Aldrich, St. Louis, USA), Dulbecco’s phosphate-buffered saline (PBS, Gibco, Waltham, USA), and trypsin (Thermo Scientific, Waltham, USA).

    Article Title: In vivo affinity maturation of the Env-binding domains of the HIV-1 entry inhibitor CD4-Ig
    Article Snippet: Before electroporation, B cells were activated for 36–42 hours in RPMI 1640 medium with 10% FBS, 100 μM Non-Essential Amino Acids (NEAA, Thermo Fisher Scientific, 11140050), 1 mM sodium pyruvate (Thermo Fisher Scientific, 11360070), 10 mM HEPES (Thermo Fisher Scientific, 15630080), 55 μM 2-Mercaptoethanol (Thermo Fisher Scientific, 21985023), 100 units/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific, 15140163), and 5 μg/ml anti-mouse CD180 antibody (Biolegend, 117708).

    Isolation:

    Article Title: The Impact of Amotosalen Photochemical Pathogen Inactivation on Human Platelet Lysate
    Article Snippet: Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium; Transfusion Innovation Center, Belgian Red Cross Flanders, Ghent, Belgium; Blood Services, Belgian Red Cross Flanders, Mechelen, Belgium; Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine and Health Sciences, Gent University, Gent, Belgium; Department of Public Health and Primary Care, Faculty of Medicine, KU Leuven, Leuven, Belgium

    Article Title: Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication
    Article Snippet: After day 9 of differentiation, cells were cultured in a medium consisting of DMEM/F12 containing 15% (v/v) Knock-Out Serum Replacement (KSR, Thermo Fisher Scientific), 1:100 (v/v) non-essential amino acids (NEAA, Thermo Fisher Scientific), 1:100 (v/v) penicillin-streptomycin (P/S, Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol (Sigma), 10 ng/mL HGF (Peprotech), and 2 ng/mL IGF-1 (Peprotech).

    Article Title: Imidazolium salt’s toxic effects in larvae and cells of Aedes aegypti and Aedes albopictus (Diptera: Culicidae)
    Article Snippet: All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM – Gibco, Thermo Fischer Scientific, Massachusetts, USA) with low levels of glucose, supplemented with 1% non-essential amino acids (NEAA – Gibco, Thermo Fischer Scientific, Massachusetts, USA), maintained at 28 °C in a 5% CO 2 incubator.

    Article Title: Proof of stability of an RSV Controlled Human Infection Model challenge agent.
    Article Snippet: Briefly, HEp2 cells (ATCC CCL-23) grown in Dulbecco’s Minimum Essential Medium (DMEM; Gibco/Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum (FBS, Tico Europe, Amstelveen, The Netherlands), 2 mM L-glutamine (Gibco/Thermo Fisher Scientific) and 1% Non-Essential Amino Acids (NEAA; Gibco/Thermo Fisher Scientific) were seeded (5 × 103 cells/well) in HEp2 medium in 96-well plates (Greiner Bio-One, Vilvoorde, Belgium) and grown until reaching 80–90% confluence.

    Article Title: Antimicrobial and immunomodulatory activities of porcine cathelicidin Protegrin-1.
    Article Snippet: Promastigotes were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal calf serum (FCS) (Bodinco B.V., Alkmaar, The Netherlands), 1x non-essential amino acids (NEAA) (Gibco), 100 units/ mL Penicillin, 100 μg/mL Streptomycin, 10 μg/mL Hemin and 10 μM Xanthine (all Sigma) at 26 ◦C.

    Article Title: Cryopreservative compositions and methods
    Article Snippet: MSCs were cultured in alpha-MEM (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with non-essential amino acids (NEAA, Gibco, Thermo Fisher Scientific, Waltham, MA) and 10% FBS (qualified, Gibco, Thermo Fisher Scientific, Waltham, MA) in a 37° C. incubator at 5% CO2.

    Article Title: Computational analysis of zoanthamine alkaloids from Zoanthus sp. as potential DKK1 and GSK-3β inhibitors for osteoporosis therapy via Wnt signaling.
    Article Snippet: The reagents and chemicals employed for cell culture comprised Minimum Essential Medium α (MEM α, Thermo Scientific, Waltham, USA), fetal bovine serum (FBS, Merck, Darmstadt, Germany), penicillin-streptomycin (Thermo Scientific, Waltham, USA), sodium pyruvate (Thermo Scientific, Waltham, USA), non-essential amino acids (NEAA, Thermo Scientific, Waltham, USA), sodium bicarbonate (Sigma-Aldrich, St. Louis, USA), trypan blue (Sigma-Aldrich, St. Louis, USA), Dulbecco’s phosphate-buffered saline (PBS, Gibco, Waltham, USA), and trypsin (Thermo Scientific, Waltham, USA).

    Article Title: In vivo affinity maturation of the Env-binding domains of the HIV-1 entry inhibitor CD4-Ig
    Article Snippet: Before electroporation, B cells were activated for 36–42 hours in RPMI 1640 medium with 10% FBS, 100 μM Non-Essential Amino Acids (NEAA, Thermo Fisher Scientific, 11140050), 1 mM sodium pyruvate (Thermo Fisher Scientific, 11360070), 10 mM HEPES (Thermo Fisher Scientific, 15630080), 55 μM 2-Mercaptoethanol (Thermo Fisher Scientific, 21985023), 100 units/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific, 15140163), and 5 μg/ml anti-mouse CD180 antibody (Biolegend, 117708).

    Modification:

    Article Title: The Impact of Amotosalen Photochemical Pathogen Inactivation on Human Platelet Lysate
    Article Snippet: Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium; Transfusion Innovation Center, Belgian Red Cross Flanders, Ghent, Belgium; Blood Services, Belgian Red Cross Flanders, Mechelen, Belgium; Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine and Health Sciences, Gent University, Gent, Belgium; Department of Public Health and Primary Care, Faculty of Medicine, KU Leuven, Leuven, Belgium

    Article Title: Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication
    Article Snippet: After day 9 of differentiation, cells were cultured in a medium consisting of DMEM/F12 containing 15% (v/v) Knock-Out Serum Replacement (KSR, Thermo Fisher Scientific), 1:100 (v/v) non-essential amino acids (NEAA, Thermo Fisher Scientific), 1:100 (v/v) penicillin-streptomycin (P/S, Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol (Sigma), 10 ng/mL HGF (Peprotech), and 2 ng/mL IGF-1 (Peprotech).

    Article Title: Imidazolium salt’s toxic effects in larvae and cells of Aedes aegypti and Aedes albopictus (Diptera: Culicidae)
    Article Snippet: All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM – Gibco, Thermo Fischer Scientific, Massachusetts, USA) with low levels of glucose, supplemented with 1% non-essential amino acids (NEAA – Gibco, Thermo Fischer Scientific, Massachusetts, USA), maintained at 28 °C in a 5% CO 2 incubator.

    Article Title: Proof of stability of an RSV Controlled Human Infection Model challenge agent.
    Article Snippet: Briefly, HEp2 cells (ATCC CCL-23) grown in Dulbecco’s Minimum Essential Medium (DMEM; Gibco/Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum (FBS, Tico Europe, Amstelveen, The Netherlands), 2 mM L-glutamine (Gibco/Thermo Fisher Scientific) and 1% Non-Essential Amino Acids (NEAA; Gibco/Thermo Fisher Scientific) were seeded (5 × 103 cells/well) in HEp2 medium in 96-well plates (Greiner Bio-One, Vilvoorde, Belgium) and grown until reaching 80–90% confluence.

    Article Title: Antimicrobial and immunomodulatory activities of porcine cathelicidin Protegrin-1.
    Article Snippet: Promastigotes were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal calf serum (FCS) (Bodinco B.V., Alkmaar, The Netherlands), 1x non-essential amino acids (NEAA) (Gibco), 100 units/ mL Penicillin, 100 μg/mL Streptomycin, 10 μg/mL Hemin and 10 μM Xanthine (all Sigma) at 26 ◦C.

    Article Title: Cryopreservative compositions and methods
    Article Snippet: MSCs were cultured in alpha-MEM (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with non-essential amino acids (NEAA, Gibco, Thermo Fisher Scientific, Waltham, MA) and 10% FBS (qualified, Gibco, Thermo Fisher Scientific, Waltham, MA) in a 37° C. incubator at 5% CO2.

    Article Title: Computational analysis of zoanthamine alkaloids from Zoanthus sp. as potential DKK1 and GSK-3β inhibitors for osteoporosis therapy via Wnt signaling.
    Article Snippet: The reagents and chemicals employed for cell culture comprised Minimum Essential Medium α (MEM α, Thermo Scientific, Waltham, USA), fetal bovine serum (FBS, Merck, Darmstadt, Germany), penicillin-streptomycin (Thermo Scientific, Waltham, USA), sodium pyruvate (Thermo Scientific, Waltham, USA), non-essential amino acids (NEAA, Thermo Scientific, Waltham, USA), sodium bicarbonate (Sigma-Aldrich, St. Louis, USA), trypan blue (Sigma-Aldrich, St. Louis, USA), Dulbecco’s phosphate-buffered saline (PBS, Gibco, Waltham, USA), and trypsin (Thermo Scientific, Waltham, USA).

    Article Title: In vivo affinity maturation of the Env-binding domains of the HIV-1 entry inhibitor CD4-Ig
    Article Snippet: Before electroporation, B cells were activated for 36–42 hours in RPMI 1640 medium with 10% FBS, 100 μM Non-Essential Amino Acids (NEAA, Thermo Fisher Scientific, 11140050), 1 mM sodium pyruvate (Thermo Fisher Scientific, 11360070), 10 mM HEPES (Thermo Fisher Scientific, 15630080), 55 μM 2-Mercaptoethanol (Thermo Fisher Scientific, 21985023), 100 units/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific, 15140163), and 5 μg/ml anti-mouse CD180 antibody (Biolegend, 117708).

    Saline:

    Article Title: The Impact of Amotosalen Photochemical Pathogen Inactivation on Human Platelet Lysate
    Article Snippet: Transfusion Research Center, Belgian Red Cross Flanders, Ghent, Belgium; Transfusion Innovation Center, Belgian Red Cross Flanders, Ghent, Belgium; Blood Services, Belgian Red Cross Flanders, Mechelen, Belgium; Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium; Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine and Health Sciences, Gent University, Gent, Belgium; Department of Public Health and Primary Care, Faculty of Medicine, KU Leuven, Leuven, Belgium

    Article Title: Acoustofluidic bioassembly induced morphogenesis for therapeutic tissue fabrication
    Article Snippet: After day 9 of differentiation, cells were cultured in a medium consisting of DMEM/F12 containing 15% (v/v) Knock-Out Serum Replacement (KSR, Thermo Fisher Scientific), 1:100 (v/v) non-essential amino acids (NEAA, Thermo Fisher Scientific), 1:100 (v/v) penicillin-streptomycin (P/S, Thermo Fisher Scientific), 0.1 mM β-mercaptoethanol (Sigma), 10 ng/mL HGF (Peprotech), and 2 ng/mL IGF-1 (Peprotech).

    Article Title: Imidazolium salt’s toxic effects in larvae and cells of Aedes aegypti and Aedes albopictus (Diptera: Culicidae)
    Article Snippet: All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM – Gibco, Thermo Fischer Scientific, Massachusetts, USA) with low levels of glucose, supplemented with 1% non-essential amino acids (NEAA – Gibco, Thermo Fischer Scientific, Massachusetts, USA), maintained at 28 °C in a 5% CO 2 incubator.

    Article Title: Proof of stability of an RSV Controlled Human Infection Model challenge agent.
    Article Snippet: Briefly, HEp2 cells (ATCC CCL-23) grown in Dulbecco’s Minimum Essential Medium (DMEM; Gibco/Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum (FBS, Tico Europe, Amstelveen, The Netherlands), 2 mM L-glutamine (Gibco/Thermo Fisher Scientific) and 1% Non-Essential Amino Acids (NEAA; Gibco/Thermo Fisher Scientific) were seeded (5 × 103 cells/well) in HEp2 medium in 96-well plates (Greiner Bio-One, Vilvoorde, Belgium) and grown until reaching 80–90% confluence.

    Article Title: Antimicrobial and immunomodulatory activities of porcine cathelicidin Protegrin-1.
    Article Snippet: Promastigotes were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 % fetal calf serum (FCS) (Bodinco B.V., Alkmaar, The Netherlands), 1x non-essential amino acids (NEAA) (Gibco), 100 units/ mL Penicillin, 100 μg/mL Streptomycin, 10 μg/mL Hemin and 10 μM Xanthine (all Sigma) at 26 ◦C.

    Article Title: Cryopreservative compositions and methods
    Article Snippet: MSCs were cultured in alpha-MEM (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with non-essential amino acids (NEAA, Gibco, Thermo Fisher Scientific, Waltham, MA) and 10% FBS (qualified, Gibco, Thermo Fisher Scientific, Waltham, MA) in a 37° C. incubator at 5% CO2.

    Article Title: Computational analysis of zoanthamine alkaloids from Zoanthus sp. as potential DKK1 and GSK-3β inhibitors for osteoporosis therapy via Wnt signaling.
    Article Snippet: The reagents and chemicals employed for cell culture comprised Minimum Essential Medium α (MEM α, Thermo Scientific, Waltham, USA), fetal bovine serum (FBS, Merck, Darmstadt, Germany), penicillin-streptomycin (Thermo Scientific, Waltham, USA), sodium pyruvate (Thermo Scientific, Waltham, USA), non-essential amino acids (NEAA, Thermo Scientific, Waltham, USA), sodium bicarbonate (Sigma-Aldrich, St. Louis, USA), trypan blue (Sigma-Aldrich, St. Louis, USA), Dulbecco’s phosphate-buffered saline (PBS, Gibco, Waltham, USA), and trypsin (Thermo Scientific, Waltham, USA).

    Article Title: In vivo affinity maturation of the Env-binding domains of the HIV-1 entry inhibitor CD4-Ig
    Article Snippet: Before electroporation, B cells were activated for 36–42 hours in RPMI 1640 medium with 10% FBS, 100 μM Non-Essential Amino Acids (NEAA, Thermo Fisher Scientific, 11140050), 1 mM sodium pyruvate (Thermo Fisher Scientific, 11360070), 10 mM HEPES (Thermo Fisher Scientific, 15630080), 55 μM 2-Mercaptoethanol (Thermo Fisher Scientific, 21985023), 100 units/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific, 15140163), and 5 μg/ml anti-mouse CD180 antibody (Biolegend, 117708).



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